What People Are Saying About Image Integrity and Datacolor CHROMACAL

CHROMACAL customers include:

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  • Amgen
  • Biogen Idec
  • Brigham and Women’s Hospital
  • Bristol-Myers Squibb
  • Case Western Reserve University
  • Delft University
  • Druquest International, Inc.
  • Gladstone Institutes
  • Indiana University Purdue University Indianapolis
  • Kansas State University
  • Leica Microsystems

  • Marine Biological Laboratory
  • National Cancer Institute
  • NeuroScience Associates
  • Sanford-Burnham Medical Research Institute
  • Seattle Children’s Hospital
  • Teva Pharmaceuticals
  • University of California, Davis
  • University of Central Missouri
  • University of Minnesota
  • University of Pennsylvania


Below is a selection of comments about the importance of image quality, color consistency, image data integrity, and the benefits of CHROMACAL:

“ChromaCal outperforms Photoshop at every key level. Unlike Photoshop, ChromaCal displays the original and adjusted specimen images side-by-side, allowing detail separation to be readily visualized in real time, especially useful in H&E stained samples….[ChromaCal] is the first tool in digital microscopy for brightfield that fulfills an industry-wide need for image standardization, and it should be in every lab that is serious about good imaging and science.”
– Gerald “Jerry” Sedgewick (consultant in the field of scientific imaging, and an expert in image forensics; author of “Scientific Imaging with Photoshop: Methods, Measurement & Output”; previously managed the University of Minnesota’s core light microscopy facility in the Department of Neuroscience)

“Having an accurate and consistent representation of the specimen is crucial in forensic analysis….CHROMACAL could not only greatly improve my analysis capabilities, but also improve collaborations with other forensic scientists, as well as provide a new standard for training of students….CHROMACAL-calibrated images are representative of good laboratory practices and ethical imaging.  This is especially important in the field of forensic science because evidence examinations must be scientifically valid to be admissible in court.”
– Gina Marie Londino, M.S. (Senior Lecturer – Forensic and Investigative Sciences Program, Indiana University Purdue University Indianapolis)

“CHROMACAL images look great!”
– A. Sally Davis, D.V.M., Ph.D. (Assistant Professor of Experimental Pathology – Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University)

“It is paramount to seek new methods for interpreting and compiling meaningful data and presenting high-quality images that pass scrutiny for publication….Not only does CHROMACAL offer major time savings, but it also provides a simple method to achieve consistency and reproducibility in my images.”
– Dawn M. Dawson, M.D. (Assistant Professor – Institute of Pathology, Case Western Reserve University)

“Color should be viewed as a standardized variable. This system [CHROMACAL] is an integrated system that encompasses the entire imaging chain. Using the CHROMACAL system I am assured that my optical system and camera are operating within the linear range. After color correction I often notice subtle differences in staining that were not readily visualized in the un-corrected micrograph. The micrographs I produce are displayed on monitors that have been calibrated using the CHROMACAL system ensuring color consistency across computer systems.”
– Daryl Goad, Ph.D. (Assistant Professor – Department of Biology and Agriculture, University of Central Missouri)

“I always say there are two ways to do imaging. One way is to create nice pictures for the cover of Nature Magazine. The other way is to create actual data. For digital pathology imaging, we are interested in actual data….The accuracy of tissue biomarker quantification requires linear image acquisition, and imaging systems are not always linear by default from the manufacturer. It is not possible to judge the accuracy of your imaging system by just looking at your digital images — you would never notice it. So, in my opinion, CHROMACAL solely as a diagnostic tool is very valuable.”
– Stefan Hamann, PhD (Biogen Idec)

[In response to two research paper retractions resulting from fabrication and falsification of reported data and images] “We—research funders, research practitioners, institutions and journals—need to put quality assurance and laboratory professionalism ever higher on our agendas, to ensure that the money entrusted by governments isn’t squandered, and that citizens’ trust in science isn’t betrayed.”
– Nature Editorial, vol. 511, pp. 5–6 (03 July 2014)

[Referring to the use of software like Photoshop] “These technologies are cheap and easy to use, but also for the panicky or unscrupulous tempting to abuse.”
– John Dahlberg, PhD (HHS Office of Research Integrity)

“CHROMACAL is KIS – keep it simple, and easy to use.  With CHROMACAL, bad camera settings, and poorly configured microscopes become obvious, and can be quickly corrected.  Scanned the CHROMACAL calibration slide with my whole slide scanner on the first try, right out of the box.”
– Stephen Hewitt, MD, PhD (National Cancer Institute)

“CHROMACAL was easy to use. I found the calibrated color to be truer and it also provided better color balance. The result was clearer color differentiation which is important for assessment and evaluation.”
– Ward R. Richter, DVM, MS, ACVP (Druquest International, Inc.)

“CHROMACAL outperforms Photoshop for both auto-white balancing and matched-brightness level. Photoshop and similar programs often over-saturate whites and rely heavily on user judgment.  CHROMACAL does it all on its own, it’s done right, and it saves time…. In addition to color calibration and auto-white balancing, CHROMACAL also includes a linearity feature that allows you to check whether your imaging system is capturing linear images.  A linear baseline is critical for scientific images, and this feature alone makes CHROMACAL a valuable tool that should be part of every microscopy facility.”
– Gerald “Jerry” Sedgewick (consultant in the field of scientific imaging, and an expert in image forensics; author of “Scientific Imaging with Photoshop: Methods, Measurement & Output”; previously managed the University of Minnesota’s core light microscopy facility in the Department of Neuroscience)