Experimental Biology 2016

April 3-5, 2016
San Diego Convention Center
San Diego, CA
Visit Datacolor in Booth #383

 Product Workshop:

“Get Your BEST Microscope Images EVER!”

Speaker: A. Sally Davis, DVM, PhD
(Kansas State University, College of Veterinary Medicine)
9:00AM, Sunday, April 3, 2016
Room A (Hall A) – far right side of Exhibit Floor

Watch the video

Exhibitor Showcase:

“Don’t Photoshop those microscope images! A new 1-click method to get consistent image quality and color.”

Speaker: Mark Clymer (Director of Marketing, Datacolor Scientific)
11:30AM, Monday, April 4, 2016
Back of Hall B – Show Floor

Best Practices for Post-Processing of Scientific Images: Photoshop and ImageJ are NOT the answer (recording)

Date presented: February 24, 2016

Speaker: Jerry Sedgewick (Imaging and Analysis, LLC)

Do you post-process your scientific images? Common tools for common adjustments include Photoshop and ImageJ, but they may not be the best answer.

The most common adjustments include:
• white balancing
• normalizing image brightness
• establishing uniformity between images (e.g. for image panels)
• correcting for uneven illumination (a.k.a. flatfield or shading correction)

During this webinar, you will see case studies involving real-world research projects that illustrate the perils of current image processing choices, and why CHROMACAL is critical for anyone involved with brightfield microscopy.

Missed the webinar?  You can view the recording here:

View Webinar Recording


ACVP/ASVCP/STP 2015 Combined Annual Meeting

October 17-20, 2015
Minneapolis Convention Center
Minneapolis, MN
Visit Datacolor in Booth #403


“Application of Color Calibration in Photomicrography for Research Pathology”

Speaker: Cathy S. Carlson, DVM, PhD, DACVP
(University of Minnesota College of Veterinary Medicine)
7:00AM, Sunday, October 18, 2015
Room: 200G (2nd floor, Minneapolis Convention Center)

Watch the Recording

Meet the Experts in Booth #403
Talk with local researchers about CHROMACAL.
When: Coffee breaks, October 18-20
Visit Booth #403 for details or to schedule an appointment.

5 Common Mistakes when using Photoshop for Science:
Tips, Tricks and a Case Study (recording)

Date presented: September 9, 2015

Speakers: Jerry Sedgewick (Imaging and Analysis, LLC) and
Dawn M. Dawson, MD (Case Western Reserve University)

Images are a vital component of scientific research for evaluation, data analysis, visualizing the impact of experimental parameters, and in medicine for assessing disease conditions. Microscope systems generally fall short in providing consistent quality images that are optimized for color, contrast and brightness. Adobe® Photoshop® or other image editing software is commonly used to correct for image insufficiencies, but consequences exist.

The speakers review the common mistakes encountered when using Photoshop for science, and offer tips — with personal experience — to achieve consistency and high quality in your imaging process, and help you avoid the 5 common mistakes altogether!

Missed the webinar? You can view the recording here:

View Webinar Recording

Image Consistency and Data Integrity in Brightfield Microscopy and Macroscopic Imaging: (recording)

A Review of ChromaCal v2.0 with expert commentary by Jerry Sedgewick

Date presented: April 30, 2015

In brightfield microscopy, color images are technically difficult to deal with. Despite attempts to manage their instruments and imaging software, researchers must accept suboptimal images (and trust that it does not affect their evaluation or analysis), or seek to correct the images with image editing software.

See how CHROMACAL v2.0 achieves image consistency and maintains the integrity of your image data. Jerry Sedgewick (Imaging and Analysis, LLC;, a leading imaging expert and consultant, provides his insights. You will also see how CHROMACAL v2.0 now supports image consistency in MACROSCOPIC reflected light environments with the new ChromaCal Macro Calibration Target.

View Webinar Recording

R u Squared?
Testing and Adjusting Imaging Systems with a Color Standard Slide (recording)

Speaker: Stefan Hamann, PhD (Biogen Idec Inc.)
Senior Scientist, Translation Pathology Laboratory

Date presented: February 12, 2015

The quantitative assessment of tissue features has become one of the hallmarks of Digital Pathology. Image analysis, however, can only be as accurate as the image acquisition that precedes it. Accuracy, linearity and long-term reliability of whole slide imaging systems are not easily user-verifiable.

This webinar will address the use of a standard color slide for quality control purposes and – when necessary – imaging system adjustments. The principles discussed in this webinar apply both to digital whole slide scanning as well as traditional digital microscope imaging.

Missed the webinar? You can register to view the recording here:

View Webinar Recording

Issues with Color in Histology in Medicine with Dr. Keith Kaplan (recording)

Date presented: November 20, 2014
Histological stains in medical and research specimens permit visual discrimination of one microscopic tissue or cellular component from another, thus enabling comparison between normal and abnormal conditions. However, much of what we see is artifact. Comprehension of tissue-level organization is the foundation to identifying and describing the abnormal/experimental situation that deviates from the normal/control situation, and this understanding heavily relies on color. But what do we do when color is inconsistent in our digital images?

Join Dr. Keith Kaplan for a review of issues with color in histology and histopathology, sources of color variation, the impact on medical and research practice, and possible directions for resolving these challenges.

Missed the webinar? You can register to view the recording here:

View Webinar Recording

Morphometry and Density/Intensity Readings: Avoid Quantification Errors (recording)

Date presented: October 21, 2014
Introduction by Michael Linden, MD, PhD (Assistant Professor, Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN)

Presenter: Jerry Sedgewick (Imaging and Analysis, LLC; author, consultant and recognized authority in post-processing, analysis and quantification of scientific images)

This webinar discusses several methods for setting thresholds when segmenting objects in images for measurement, but inconsistent color and contrast remain hurdles to high image quality and confident quantization. Learn how to create consistent images, without subjective adjustments, for subsequent quantization. Learn how to confirm your imaging system delivers scientific images that are linear. Learn how to standardize the color in your images, while maintaining linearity, for greater confidence in quantitative analysis.”

Missed the webinar? You can view the recording here:

View Webinar Recording

A Better Choice than Photoshop… Webinar (recording)

Date presented: September 10, 2014
It’s frustrating – you spend weeks on your study or experiment, finally taking images on the microscope, but the color just isn’t right. The common choice was to use Photoshop to correct the images produced from digital microscope cameras, until now…

Jerry Sedgewick (Imaging and Analysis, LLC), a former core microscopy facility manager, is a recognized authority in applying Photoshop to edit scientific images. Through his books, articles and workshops, Mr. Sedgewick has helped hundreds of researchers use Photoshop to achieve their desired results. So why does he now feel there is a better approach for brightfield microscopy? Mr. Sedgewick will review his recommended practices in Photoshop, and then demonstrate how CHROMACAL can deliver an improved result with better controls and less time for assured image quality.

Missed the webinar? You can register to view the recording here:

View Webinar Recording

Image Integrity Webinar (recording)

How Image Integrity Impacts Your Ability to Publish… or Perish.

Date presented: June 11, 2014
Retractions of papers from publication have increased tenfold between 2005 and 2011. Each incident affects the reputation of scientists, academic institutions, and public perception, which can lead to reduced funding from NIH and NSF. The role of images in publication has largely been a subtopic in Publication Ethics, yet reported cases of scientific misconduct in which images have been involved ballooned to 80% from 1989 to 2008. Images require a series of specific steps to be taken in order to protect, calibrate and create publication-ready images in research and reports. These will be covered in the seminar “How Image Integrity Impacts Your Ability to Publish… or Perish.”

Presenter: Jerry Sedgewick, Image and Analysis, LLC
Missed the webinar? You can register to view the recording here:

View Webinar Recording

CHROMACAL Webinar (recording)

Date presented: April 2, 2014
This webinar reviews best practices in brightfield microscopy imaging, and how Datacolor CHROMACAL ensures image quality, adds greater confidence and consistency in color reproduction in your digital specimen images. Introduction by Eduardo Rosa-Molinar, PhD, Professor of Biology, University of Puerto Rico – Rio Piedras.

Missed the webinar? You can register to view the recording here.

View webinar recording