Video Library

Overviews Webinars Tutorials


What can Datacolor CHROMACAL do for you?

Do you know what Datacolor CHROMACAL can do for you? This introductory video explains how CHROMACAL establishes and communicates standardized color images. The CHROMACAL result delivers digital imaging quality and integrity that matches the rigor of your science.

CHROMAflash: See what’s new in ChromaCal v2.0

This short video introduces you to the latest features available in the release of ChromaCal v2.0. You’ll see new support for macroscopic images, as well as new image viewing capabilities. A new export function for calibration matrix statistics is a “must have” for users of whole slide imaging systems where performance is critical.

CHROMAflash: Overview of ChromaCal Image Calibration Software

This CHROMAflash video illustrates the ease and speed at which you can achieve consistent, standardized color in your TIFF and JPEG specimen images using Datacolor ChromaCal.

CHROMAflash: Linearity – why it matters and troubleshooting

In this brief CHROMAflash video, you’ll be introduced to image linearity and why you should pay attention to it. You’ll also see how ChromaCal allows you to check and manage the linearity of your imaging system for consistent and comparable results. Click here for a pdf version of the presentation slides.

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Get your BEST microscope images EVER! (Apr 3, 2016)

Speaker: : A. Sally Davis, DVM, PhD (College of Veterinary Medicine, Kansas State University) and Mark Clymer (Datacolor Inc.)

How do I get the best images from my microscope system?

My images aren’t quite right. How do I adjust them?
Join us for a discussion of best practices in brightfield microscopy. This workshop will review and reinforce the imaging processes and post-acquisition processing steps to deliver the best microscope images possible. In this workshop you will learn about microscopy and imaging system techniques, image processing options, and best practices for establishing consistent microscope images for analysis and presentation. Discussion will be augmented with real research examples.

Best Practices for Post-Processing of Scientific Images: Photoshop and ImageJ are NOT the answer (Feb 24, 2016)

Speaker: Jerry Sedgewick (Imaging and Analysis, LLC)

Do you post-process your scientific images? Common tools for common adjustments include Photoshop and ImageJ, but they may not be the best answer.

The most common adjustments include:

  • white balancing
  • normalizing image brightness
  • establishing uniformity between images (e.g. for image panels)
  • correcting for uneven illumination (a.k.a. flatfield or shading correction)

During this webinar video, you will see case studies involving real-world research projects that illustrate the perils of current image processing choices, and why CHROMACAL is critical for anyone involved with brightfield microscopy.

Application of Color Calibration in Photomicrography for Research Pathology (Oct 18, 2015)

Speaker: Cathy S. Carlson, DVM, PhD, DACVP (College of Veterinary Medicine, University of Minnesota)

In research pathology, it is critical that color in photomicrographs and gross images accurately represents what is seen in the specimen. Consistency between images and image color rendering on computer monitors is essential for viewing and evaluating images. Several case studies (both microscopic and macroscopic) illustrate the importance of color calibration to assessments in research studies.

This video is a recording of the morning seminar delivered by Dr. Carlson at the ACVP/ASVCP/STP 2015 Combined Annual Meeting in Minneapolis, MN. Dr. Carlson comments about the poor image reproduction by the LCD projector in the seminar room, but this video was recorded directly from the PC, thus providing accurate image color and avoiding the issues described by Dr. Carlson. Enjoy!

5 Common Mistakes when using Photoshop for Science: Tips, Tricks and a Case Study (Sep 9, 2015)

Guest speakers: Jerry Sedgewick (Imaging and Analysis, LLC) and Dawn M. Dawson, MD (Case Western Reserve University)

Images are a vital component of scientific research for evaluation, data analysis, visualizing the impact of experimental parameters, and in medicine for assessing disease conditions.  Microscope systems generally fall short in providing consistent, quality images that are optimized for color, contrast and brightness.  Adobe® Photoshop® or other image editing software is commonly used to correct for image insufficiencies, but consequences exist.

The speakers will review the common mistakes encountered when using Photoshop for science, and offer tips — with personal experience — to achieve consistency and high quality in your imaging process, and help you avoid the 5 common mistakes altogether!

R u Squared? Testing and Adjusting Imaging Systems with a Color Standard Slide (Feb 12, 2015)

Guest speaker: Stefan Hamann, PhD – Senior Scientist, Translation Pathology Laboratory at Biogen Idec.

The quantitative assessment of tissue features has become one of the hallmarks of Digital Pathology. Image analysis, however, can only be as accurate as the image acquisition that precedes it. Whole slide imaging and scanner systems are now offered by a variety of vendors and these instruments differ in speed, degree of automation, image quality and consistency, slide capacity and, last but not least, price. However, accuracy, linearity and long-term reliability are not easily user-verifiable. This webinar will address the use of a standard color slide for quality control purposes and – when necessary – imaging system adjustments. The principles discussed in this webinar apply both to digital whole slide scanning as well as traditional digital microscope imaging.

Issues with Color in Histology in Medicine (Nov 20, 2014)

Histological stains in medical and research specimens permit visual discrimination of one microscopic tissue or cellular component from another, thus enabling comparison between normal and abnormal conditions. However, much of what we see is artifact. Comprehension of tissue-level organization is the foundation to identifying and describing the abnormal/experimental situation that deviates from the normal/control situation, and this understanding heavily relies on color. But what do we do when color is inconsistent in our digital images?

Morphometry and Density/Intensity Readings: Avoid Errors in Quantification (Oct 21, 2014)

Originally airing on October 21, 2014, this webinar discusses several methods for setting thresholds when segmenting objects in images for measurement, but inconsistent color remains a hurdle to confident quantization. Learn how to create consistent images, without subjective adjustments, for subsequent quantization. Learn how to confirm your imaging system delivers scientific images that are linear. Learn how to standardize the color in your images, while maintaining linearity, for greater confidence in quantitative analysis. Michael Linden, MD, PhD (University of Minnesota) delivers the introduction and Jerry Sedgewick (Imaging and Analysis) is the presenter.

A Better Choice than Photoshop (Sep 10, 2014)

Originally presented on September 10, 2014, this webinar “A Better Choice than Photoshop for Color Brightfield Microscope Images” explores and demonstrates the common uses of Photoshop to adjust color in scientific images, and then demonstrates better results using ChromaCal. Watch the video and see why a leading authority in using Photoshop for scientific images now feels that ChromaCal is the better option.

How Image Integrity Impacts Your Ability to Publish…or Perish (Jun 11, 2014)

Originally presented as a webinar on June 11, 2014, the video “How Image Integrity Impacts Your Ability to Publish… or Perish” reviews the unsettling trend in scientific paper rejections, and introduces guidance on image editing processes to avoid potential rejection and improve potential acceptance of your scientific images for publication.

Ensuring Color Integrity with Datacolor CHROMACAL (Apr 2, 2014)

Originally presented as a webinar on Apr 2, 2014, the video “Ensuring Color Integrity with Datacolor CHROMACAL” reviews best practices in brightfield microscopy imaging, sources for color variability in images, and how Datacolor CHROMACAL adds greater confidence and consistency in color reproduction in your digital specimen images.

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Aligning the calibration matrix image in ChromaCal

Calibrate your color brightfield images with CHROMACAL to maintain color integrity. In this video, you’ll learn how to load a calibration slide image. You will then see how to calibrate a single specimen image, or how to batch process multiple images.

CHROMACAL Image Calibration Software

Calibrate your color brightfield images with CHROMACAL to maintain color integrity. In this video, you’ll learn how to load a calibration slide image. You will then see how to calibrate a single specimen image, or how to batch process multiple images.

How to Capture a CHROMACAL Color Calibration Slide

This video demonstrates how to capture an image of the CHROMACAL color calibration slide for transmitted brightfield microscopy. The calibration slide image would then be used with the CHROMACAL color calibration software for brightfield images. Watch the CHROMACAL Image Calibration Software videos to see how the calibration slide image is used.

Good Microscopy Practices

A quick refresher to setting up your microscope for digital color imaging. Review Koehler illumination and camera settings for improved consistency in your imaging experiments.

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